Protein Method Blocking Buffers
LI COR BIOTECH LLC Intercept T20 (TBS) Protein-Free Antibody Diluent (500 mL)
Intercept T20 (TBS) Protein-Free Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (TBS) Protein-Free Blocking Buffer in Tris-buffered saline (TBS) preformulated with Tween 20. This diluent can be used where animal-based products are prohibited. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (TBS) Protein-Free Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
LI COR BIOTECH LLC Intercept (TBS) Blocking Buffer and Diluent Kit
Try Intercept (TBS) Blocking Buffer and Intercept T20 (TBS) Antibody Diluent for improved results in your quantitative Western blots and other immunoassays. Intercept Blocking Buffers provide excellent blocking performance with low background and low variability. Intercept T20 Antibody Diluents improve the specificity of the primary and secondary antibodies, reducing off-target effects. They are preformulated with Tween 20. There’s no need to mix the diluent yourself, which saves you time and reduces potential variation. This kit includes Intercept (TBS) Blocking Buffer (1 x 500 mL) and Intercept T20 (TBS) Antibody Diluent (2 x 500 mL)
LI COR BIOTECH LLC Intercept (PBS) Blocking Buffer (500 mL)
Intercept (PBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (PBS) Blocking Buffer is the blocking buffer of choice for near-infrared fluorescent applications when using a phosphate-buffered saline (PBS) system. It contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies, it is an excellent choice for biotin-streptavidin detection and it is suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays. Intercept (PBS) Blocking Buffer is a ready-to-use blocker formulation in PBS. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection. It is stored at 4 °C and is also available in kits with Immobilon-FL PVDF and nitrocellulose membranes.
Teknova 20X PBS with 1% Tween-20. 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
LI COR BIOTECH LLC Intercept T20 (TBS) Antibody Diluent (500 mL)
Intercept T20 (TBS) Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (TBS) Blocking Buffer in Tris-buffered saline (TBS) preformulated with Tween 20. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (TBS) Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
Vector Laboratories Mouse On Mouse (M.O.M.) Blocking Reagent 1 ml
M.O.M. Mouse Ig Blocking Reagent is used to block endogenous mouse antibody in tissue sections. A component of the M.O.M. Kits, this reagent provide optimal staining results when it is paired with the M.O.M. Biotinylated Anti-Mouse Ig Reagent or the biotin-free, one-step micropolymer detection reagent, ImmPRESS Anti-Mouse Ig Reagent (MPX-2402). This reagent is supplied as a 1 ml concentrate, sufficient to prepare about 25 ml of working solution, or enough to stain 125-250 sections.
Biotium 10X Fish Gelatin Blocking Agent
10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or used for blocking and dilution of antibodies during immunostaining procedures.
LI COR BIOTECH LLC Intercept (TBS) Protein-Free Blocking Buffer (500 mL)
Intercept (TBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays where animal-based products are prohibited. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no proteins, for assays where animal-based products are prohibited, it can be used with the widest range of targets for Western blot detection, it is ideal for primary antibodies that are optimized for Tris-based buffer systems, it can be used for detection of phosphorylated targets, it has superior performance for alkaline phosphatase detection, and it is an excellent choice for biotin-streptavidin detection. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in TBS and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
Teknova 10mM Tris-HCl with 0.1% Tween-20, pH 8.5. 1mL/Tube, 50 Pack, Sterile.
10mM Tris-HCl, 0.1% Tween-20
LI COR BIOTECH LLC Intercept T20 (PBS) Antibody Diluent (500 mL)
Intercept T20 (PBS) Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects.The diluent contains Intercept (PBS) Blocking Buffer preformulated with Tween 20 for use in a phosphate-buffered saline (PBS) system. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (PBS) Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.