Protein Method Blocking Buffers
Abcam 10 x Blocking Buffer, 50ML
10X blocking buffer (pH 7.5) for serum-free blocking of non-specific antibody binding. - Cited in over 30 publications - Suitable for ELISA, in-cell ELISA, ICC/IF, western blot (WB) - 100% casein blocking as preservative
The product is subject to the following: Abcam Restricted Use Statement
LICORbio Intercept T20 (TBS) Antibody Diluent (500 mL)
Intercept T20 (TBS) Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (TBS) Blocking Buffer in Tris-buffered saline (TBS) preformulated with Tween 20. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (TBS) Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
Bio Rad Laboratories EveryBlot Blocking Buffer, 500 mL
EveryBlot Blocking Buffer provides 5 minute blocking and maximum sensitivity for all western blots regardless of detection method.
Teknova 20X PBS with 1% Tween-20. 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
LICORbio Intercept (TBS) Blocking Buffer (3 x 500 mL)
Intercept (TBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (TBS) Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies; it can be used with the widest range of targets for Western blot detection; it is ideal for primary antibodies that are optimized for Tris-based buffer systems; it can be used for detection of phosphorylated targets; it has superior performance for alkaline phosphatase detection; it is an excellent choice for biotin-streptavidin detection.Shake well before each use.
LICORbio Intercept (TBS) Protein-Free Blocking Buffer (500 mL)
Item is non returnable
Intercept (TBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays where animal-based products are prohibited. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no proteins, for assays where animal-based products are prohibited, it can be used with the widest range of targets for Western blot detection, it is ideal for primary antibodies that are optimized for Tris-based buffer systems, it can be used for detection of phosphorylated targets, it has superior performance for alkaline phosphatase detection, and it is an excellent choice for biotin-streptavidin detection.
Sigma Aldrich Fine Chemicals Biosciences Phosphate Buffered Saline with 0.05% TWEEN 20, pH 7.4, 10 Pack
Phosphate Buffered Saline with 0.05% TWEEN 20, pH 7.4, 10 Pack
SCANTIBODIES LABORATORY INC HBR 1, PURIFIED (3KC533) 500MG
The 3KC533 Heterophilic Blocking Reagent 1, Purified from Scantibodies Laboratory, Inc. contains specific murine immunoglobulins that block the heterophilic interaction by active binding to the heterophilic antibodies, which are capable of cross-linking the capture and the detection antibodies used in the immunoassay, resulting in false positive readings. The attachment of HBR-1 to the heterophilic antibodies blocks this cross-linking, and eliminates the interference caused by the heterophilic antibodies in the humoral fluids. In addition to its active blocking, this product is also characteristic of its passive blockage of the heterophilic interaction as well. The HBR is a liquid reagent with a protein concentration of 20 ± 2 mg/ml. The immunoglobulins are dissolved in a phosphate buffer with a pH of 7.2-7.6. The immunoglobulins in this product are at a purity of greater than or equal to 95%.
Vector Laboratories Mouse On Mouse (M.O.M.) Blocking Reagent 1 ml
M.O.M. Mouse Ig Blocking Reagent is used to block endogenous mouse antibody in tissue sections. A component of the M.O.M. Kits, this reagent provide optimal staining results when it is paired with the M.O.M. Biotinylated Anti-Mouse Ig Reagent or the biotin-free, one-step micropolymer detection reagent, ImmPRESS Anti-Mouse Ig Reagent (MPX-2402). This reagent is supplied as a 1 ml concentrate, sufficient to prepare about 25 ml of working solution, or enough to stain 125-250 sections.
Surmodics Inc StabilBlock Immunoassay Stabilizer, 1L
StabilBlock Immunoassay Stabilizer effectively preserves the conformation and activity of dried proteins while simultaneously blocking the surface to reduce non-specific binding. It can be used to stabilize antibodies, antigens or enzymes on an assortment of immunoassay components including polystyrene plates, tubes, glass, membranes, and filter paper. The key benefits are reduction of non-specific binding while increasing signal-to-noise ratios within immunoassay applications.
Biotium TrueBlack Plus Lipofuscin Autofluorescence Quencher | 500uL
TrueBlack Plus Lipofuscin Autofluorescence Quencher | 40x concentrated solution in DMSO | 500uL
LICORbio Intercept T20 (TBS) Antibody Diluent (3 x 500)
Intercept T20 (TBS) Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (TBS) Blocking Buffer in Tris-buffered saline (TBS) preformulated with Tween 20. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (TBS) Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
Meridian Life Science TRU Block ULTRA, 0.1 g, Liquid, Immunoassay interference blocker, >95% purity, 24-26 mg/mL protein concentration, 10 mM Sodium Phosphate, 150 mM Sodium Chloride
TRU Block ULTRA, 0.1 g, Liquid, Immunoassay interference blocker, >95% purity, 24-26 mg/mL protein concentration, 10 mM Sodium Phosphate, 150 mM Sodium Chloride